Insertion junction: CLIP2.053112_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.053112
Chromosome:chromosome 6
Location:5200827
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g283750 HST1 (1 of 1) K12501 - homogentisate solanesyltransferase (HST); Homogentisate solanesyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGACTGGGCACTGCTGCCGAAGGCGCTGCTCGGCCTGGTGGCGCTGCTG
Internal bar code:GTTAGCGGTGGAAAGAGTGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1085
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TCCACACACACACACACACA
Suggested primer 2:AATTGCACGCGCTTTCTTGT