Insertion junction: CLIP2.053113_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.053113
Chromosome:chromosome 12
Location:2662219
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g505400 (1 of 1) 3.1.1.47 - 1-alkyl-2-acetylglycerophosphocholine esterase / Platelet-activating factor acetylhydrolase; Related to platelet-activating factor acetylhydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCTCAGCTGCCCCAACGCACCCACCCACCCACGCATTCTCCGTCAAAC
Internal bar code:GGGGTACGGGAGAGAAAAATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:4547
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:78
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:78

Suggested primers for confirmation by PCR

Suggested primer 1:GCACGCACACAAGAACCATT
Suggested primer 2:CACTCCTCCGGGAAACAGTC