| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.053118 |
| Chromosome: | chromosome 17 |
| Location: | 4052806 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728650 | FAP196 | (1 of 2) PTHR13720//PTHR13720:SF14 - WD-40 REPEAT PROTEIN // WD REPEAT-CONTAINING PROTEIN 16; Flagellar Associated Protein 196 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCCAAGCACGGCCTGGAGCCCGGCAAGGTGAGGGCGGCGCATGGAACT |
| Internal bar code: | GGATTTATGCAGGCCTTAGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 696 |
| LEAP-Seq percent confirming: | 10.6383 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGGACTCGTGACCCCATC |
| Suggested primer 2: | ATACCTTTGTGCCCGGTGAG |