Insertion junction: CLIP2.053129_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.053129
Chromosome:chromosome 2
Location:239634
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g074626 CSB11 (1 of 1) IPR000104//IPR010095//IPR013083 - Antifreeze protein, type I // Transposase IS605, OrfB, C-terminal // Zinc finger, RING/FYVE/PHD-type; {"Probable transposon-derived protein of Chlamydomonas-Specific family B intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACTCAACCCACCCACCTTGGAGGTCTTGTATTCATCGATGATGAACA
Internal bar code:CGGTTAGTACGATCTTGAATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1369
LEAP-Seq percent confirming:68.5714
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCACAGTTGTCAACAGCC
Suggested primer 2:CGTTATCAACCCAGCCACCT