Insertion junction: CLIP2.053132_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.053132
Chromosome:chromosome 12
Location:8606685
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g548450 GT90-24,GT90F24 (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 24 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGTGTGGGGGCAGCTGGAGGGGTGGACATGGGTGGAGGGTGCACAAGG
Internal bar code:ATGAATGCAGGCCGTGACTAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:356
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTGTTCCCTCACGCACAT
Suggested primer 2:GAAAGTGAGGAAGACCCCGG