| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.053138 |
| Chromosome: | chromosome 4 |
| Location: | 3462178 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g800492 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTCCAAGTGGCGCTGCCGAGAGTACAGAGCCTCGCGCTCCTGCACCC |
| Internal bar code: | TAGGTGGGCCTTTTCTATAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3240 |
| LEAP-Seq percent confirming: | 82.8571 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGTCTTGGGCACTTGCGT |
| Suggested primer 2: | GATGATGCTCCCGTTGTTGC |