| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.053156 |
| Chromosome: | chromosome 3 |
| Location: | 6149169 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g190800 | TMPK1,DIV28 | dTMP kinase; (1 of 2) 2.7.4.9 - dTMP kinase / TMPK | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCCCCTCCGTGCTCGAATCTAGCACGCACTCACCCCTTGGCTCTTGA |
| Internal bar code: | GAGTTTCTTGCTTGCAATGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 75 |
| LEAP-Seq percent confirming: | 7.69231 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGAAGGGGGAAATGTCG |
| Suggested primer 2: | AATCTGATTGGGGCTGTGGG |