| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053161 |
| Chromosome: | chromosome 3 |
| Location: | 5215216 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| lncRNA_TCONS_00234913 |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACCGAGGAATGGGGAGCCATCACGGGACATTGACTAGGACACAGTGAT |
| Internal bar code: | AGTGCTATGCGTTTTTCGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3586 |
| LEAP-Seq percent confirming: | 8.33333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAGAGGACCGTTCTAGC |
| Suggested primer 2: | CGGGGATGCCGCATTTTAAG |