| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.053174 |
| Chromosome: | chromosome 14 |
| Location: | 499552 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611200 | (1 of 10) PF02536 - mTERF (mTERF) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTTATTGCCTTGCTGGCGCGTTCCAACGGGCCCGCGTATCGCTCCGCA |
| Internal bar code: | GTACATGTACTAGGGGAATCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1031 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGTACGATAGGAACAGGG |
| Suggested primer 2: | GCAGAAAGCAGGTTTGGGTG |