Insertion junction: CLIP2.053187_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.053187
Chromosome:chromosome 9
Location:2332494
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g394350 NUP155 Nucleoporin 155; (1 of 1) K14312 - nuclear pore complex protein Nup155 (NUP155, NUP170, NUP157) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCGTGCCACTCCGTTGTCAAGCATTGGCCACGCTCTCCTCCTCGCTG
Internal bar code:AGCCGACTTTTCCTAGTGGTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1222
LEAP-Seq percent confirming:91.3043
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:AGTGTTCCTGCCAGCCATAC
Suggested primer 2:GTAGCCACCATGAAGCAGGT