| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053197 |
| Chromosome: | chromosome 9 |
| Location: | 3062984 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393876 | (1 of 3) 2.1.1.200 - tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferase / TrMet(Xm32) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTGCTTTGGGGCCACTAGCACCACCCGAACGTCCGACAGCAGCAGGT |
| Internal bar code: | GCGCGTAAGACTTTTTACTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1595 |
| LEAP-Seq percent confirming: | 78.125 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGGCTGTTGTAACGCAA |
| Suggested primer 2: | TCACAACCACAACCAGACCC |