| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.053204 |
| Chromosome: | chromosome 16 |
| Location: | 5218950 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684550 | HAR2 | Possible 3-hydroxyacid dehydrogenase; (1 of 3) 1.1.1.26 - Glyoxylate reductase / NADH-dependent glyoxylate reductase | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCGGCGATCCGCTCCGTCCTTATGGAAGACCCGTCCGTGCGCCCCCA |
| Internal bar code: | TTGCCGGGCGTGTTCTTTATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 754 |
| LEAP-Seq percent confirming: | 44.8276 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGCGATGCAATGAGCTG |
| Suggested primer 2: | CGGAAACATCGCTGTCGTTC |