| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053206 |
| Chromosome: | chromosome 7 |
| Location: | 2553395 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329882 | VGK1,VGKC | Ion transport protein; (1 of 1) PTHR10217//PTHR10217:SF503 - VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATGATGGCTCAAATCCTTCGAGAGCTTGACTCGCTGCGAACACAGCAA |
| Internal bar code: | TTACTAATAGGGTGGGTTGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3581 |
| LEAP-Seq percent confirming: | 41.3333 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGTCCCAATGAACTCGAA |
| Suggested primer 2: | GAATGGATGCGACATTCGGC |