| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.053224 |
| Chromosome: | chromosome 17 |
| Location: | 721957 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g700750 | GFY3 | (1 of 5) PTHR30178 - INNER MEMBRANE PROTEIN YAAH; Putative acetate transporter | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGTCCTTAATCGGGGCCATAGCCACGAGCTGGGCCTTGGTCTTGGCGC |
| Internal bar code: | GAGGTTTGACCTTCTATCTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2389 |
| LEAP-Seq percent confirming: | 90.1408 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCGTGTCCTTCTTCAGGG |
| Suggested primer 2: | CAGTGTTGCAACGGTGTGAG |