| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.053228 |
| Chromosome: | chromosome 2 |
| Location: | 949703 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g079351 | 5'UTR | ||
| Cre02.g800176 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCGCCAAGCCCAGAGCCGCCGTCACCAGCACCACCATTCCCGCCGTCC |
| Internal bar code: | GGTCGATATGGATTCAAGATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1172 |
| LEAP-Seq percent confirming: | 86.9565 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGAAGGAGGAGCAGGAGA |
| Suggested primer 2: | CTTCGCCTGCTCCTACTGTG |