Insertion junction: CLIP2.053249_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.053249
Chromosome:chromosome 2
Location:1732431
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g086200 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTAGGCGCGGCAGCGTAAGGGATGGCGGCCGCGGCAGACTAAGCCAGGGT
Internal bar code:AATTAATGTATAAAGTGAGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1308
LEAP-Seq percent confirming:95.2381
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CACGTGACCACAACTAGGCT
Suggested primer 2:ACGTTCTTCCCGGCTGTAAG