Insertion junction: CLIP2.053250_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.053250
Chromosome:chromosome 7
Location:3489346
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g336000 (1 of 3) 3.6.1.22//3.6.1.55 - NAD(+) diphosphatase / NADP pyrophosphatase // 8-oxo-dGTP diphosphatase / 8-oxo-dGTPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTGCATCATCGGTGGGTTCTAGCGGTCAGCACAGATCTTCCCCTAGCC
Internal bar code:CATATTGTTATGTCTTTTCATG

Confirmation - LEAP-Seq

LEAP-Seq distance:2661
LEAP-Seq percent confirming:97.7778
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:AAAGGGCGAAGGTCAGGAAG
Suggested primer 2:CATCGGTTGCGCCCAATAAG