| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053251 |
| Chromosome: | chromosome 13 |
| Location: | 998421 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g568400 | FHL4,YME1,FTSH4 | FtsH-like ATPase/metalloprotease; (1 of 1) K08955 - ATP-dependent metalloprotease [EC:3.4.24.-] (YME1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACTCCGAGCCGGAGGTGTAGAAGAAGGGCACACCGGCCTCGCCTATGT |
| Internal bar code: | AACATTTCAAGCGTTCCTACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1534 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGGTTACAGGCAGGGAG |
| Suggested primer 2: | CACCTTGAATCCGTCCAGCT |