| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053259 |
| Chromosome: | chromosome 3 |
| Location: | 5626778 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g186000 | (1 of 1) PF08670 - MEKHLA domain (MEKHLA) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTCCCACACCCGAGGAGATTGAGGCGGCGGAGGCGGCTGTGGCGGAGC |
| Internal bar code: | GACGGTTTATGCGATTTACAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4259 |
| LEAP-Seq percent confirming: | 86.0 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATCTGGACTGGGATGTCG |
| Suggested primer 2: | CACCTCTCGTTGCCTCATGT |