| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.053279 |
| Chromosome: | chromosome 2 |
| Location: | 6886455 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388726 | (1 of 1) 3.1.3.32 - Polynucleotide 3'-phosphatase / 2'(3')-polynucleotidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCGCGTCCTGGTTGTCAGTTATGAAGGCCGCGCGCCACCTTGGTGCGC |
| Internal bar code: | TGGTAGATGTTGAAGTTTGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3864 |
| LEAP-Seq percent confirming: | 53.3333 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCATCGACTCCATCATGGA |
| Suggested primer 2: | TAGCACACGGTTCTCACACC |