Insertion junction: CLIP2.053282_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.053282
Chromosome:chromosome 11
Location:3877119
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g479650 CHIP,CHIP1 (1 of 1) K09561 - STIP1 homology and U-box containing protein 1 (STUB1, CHIP); Carboxyl terminus of Hsc70-interacting protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACTCCCGCACCGAAGTCCACACCCACATCCACACTCACACCCGCCCACG
Internal bar code:CCAATTAGTACTCTGTGTTTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2903
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:79
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:79

Suggested primers for confirmation by PCR

Suggested primer 1:CTCAACCTCCACCACTGGAC
Suggested primer 2:TATTTGTTTGTTGCGGCGCA