| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053294 |
| Chromosome: | chromosome 17 |
| Location: | 1682464 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g802043 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGAGGGGTTTTGGGCCACGGTTAAGGTGCGTCTGGGTTCGGCAAGGGCT |
| Internal bar code: | AAAAAGGATAGGCGTTATAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3453 |
| LEAP-Seq percent confirming: | 7.40741 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCTACAGCCGACTCCTA |
| Suggested primer 2: | TTTGCTGACGTGTAGCGACT |