| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053323 |
| Chromosome: | chromosome 9 |
| Location: | 4608093 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401589 | (1 of 2) IPR000104//IPR002893//IPR003072 - Antifreeze protein, type I // Zinc finger, MYND-type // Orphan nuclear receptor, NOR1 type | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCCCTGGCGGCTGGCGCGCCGCAACCGTCAGCACGGGCCAGCAGTTA |
| Internal bar code: | ACGTGTACTTAAGGGGGCTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2729 |
| LEAP-Seq percent confirming: | 94.5946 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCTCCCTGGTATGGCATC |
| Suggested primer 2: | CTTGTGCCCTTGTGGAAACG |