Insertion junction: CLIP2.053328_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.053328
Chromosome:chromosome 3
Location:3873353
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g170550 POC4 Proteome of centriole protein 4 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCATACCTAGGTTGCGCGCAAGGCAGCGCACGTGTTTGGGGCGACGCGA
Internal bar code:ATACAAGGATGTTTCGCGAAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1161
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GGCTGGTACTTACAACCCCC
Suggested primer 2:CTACTGCGCTACACCTACCG