| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053336 |
| Chromosome: | chromosome 3 |
| Location: | 8072372 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201301 | (1 of 2) IPR000104//IPR000945//IPR020683 - Antifreeze protein, type I // Dopamine beta-hydroxylase-related // Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGACCGCGGGTGGTGCTGCGGCTGCGCCAGCTGCGTTGGCGGTGGCTGG |
| Internal bar code: | TCTGAGGCAGGAGCATGGGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 885 |
| LEAP-Seq percent confirming: | 56.25 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCACGGACACATCCATTG |
| Suggested primer 2: | TTGTGTGCAGGTGTGTGAGT |