| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053350 |
| Chromosome: | chromosome 5 |
| Location: | 3512732 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g800579 | (1 of 352) PF00078 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTTATGCGGATGACGTTAGCTTACTAGCCACCTCCTTTGAACGGATGC |
| Internal bar code: | GTTATTGTGGCCCTTCCTGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2203 |
| LEAP-Seq percent confirming: | 19.2308 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCAGTACACTGCCTCAT |
| Suggested primer 2: | CAGGCGACCCCCTTAACATT |