Insertion junction: CLIP2.053351_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.053351
Chromosome:chromosome 11
Location:311861
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467567 UBQ1 Ubiquitin; (1 of 1) PTHR10666:SF150 - POLYUBIQUITIN 3 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCGAAGACTCCACCTCGAGCGTGATGGTCTTGCCCGTCAGGGTCTTGA
Internal bar code:TGTCTTTGCGTCCTGTTTTAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2171
LEAP-Seq percent confirming:53.8462
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:CACTGGGCTGTGTTGATTGC
Suggested primer 2:CTGGATCTTGGCCTTCACGT