| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.053352 |
| Chromosome: | plastome |
| Location: | 126725 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802316 | ChreCp052,2717043,ycf10,cemA | (1 of 1) PTHR33650:SF2 - CHLOROPLAST ENVELOPE MEMBRANE PROTEIN; heme-binding protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCCGATTTCTTCATAAGTAATTGAAACTACTTTATCTTTATCTGTATA |
| Internal bar code: | ACAGGGCTTACCATAATACTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 157 |
| LEAP-Seq percent confirming: | 78.5714 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGACCCTGCTCGTAACCC |
| Suggested primer 2: | ATTTCACCCTCTGCGGAAGG |