Insertion junction: CLIP2.053355_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.053355
Chromosome:chromosome 13
Location:870241
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g567450 HON1 Histone H1; (1 of 3) K11275 - histone H1/5 (H1_5) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGACGCCTCCTTCTTGGGGGCCTTGGCCTTAGGGGCCTTCGGCTTGGC
Internal bar code:TTTGAGGTTCAAGGGATGGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:3644
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:55
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:55

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCAAATGGTTGGCTTGTC
Suggested primer 2:AAAGTCTTGATGGCCAGCGA