Insertion junction: CLIP2.053363_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.053363
Chromosome:chromosome 11
Location:3555354
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g477850 TXC1,TSN1 (1 of 1) K15979 - staphylococcal nuclease domain-containing protein 1 (SND1); Transcriptional coactivator-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCTTGCCGGCGTCGCGGACCTGAACAGCGGGGAGGTTGCAAAGATTGG
Internal bar code:GGGTCTCAGTTTGTTTTGTCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:535
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCAACACGGTTTTAGCA
Suggested primer 2:GGGGGTTGCAAGGCTAGAAT