| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.053365 |
| Chromosome: | chromosome 3 |
| Location: | 533427 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145147 | TRP8 | Transient receptor potential ion channel protein; (1 of 6) PTHR31942//PTHR31942:SF1 - FAMILY NOT NAMED // MLO-LIKE PROTEIN 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGCTGGAAACCCCATCCATCCCCATCCCCACCAATCCATGCCACTGC |
| Internal bar code: | CAAGCCTGCTGACTAGTCTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1922 |
| LEAP-Seq percent confirming: | 77.1429 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGCTGCGTTGACTTTCA |
| Suggested primer 2: | CATCATCGTGCTGCACTTCG |