Insertion junction: CLIP2.053368_4

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.053368
Chromosome:chromosome 12
Location:693828
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g492950 NSG5,DIV15,RIR1 (1 of 1) K10807 - ribonucleoside-diphosphate reductase subunit M1 (RRM1); Ribonucleoside-diphosphate reductase R1 subunit intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGCCGACCATGCGCTCACTCGGCTTCCACCCTGTCCTCCCGGTCCGCA
Internal bar code:GGTATGCTCTAAAAACCATTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:6103
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:91
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:91

Suggested primers for confirmation by PCR

Suggested primer 1:CCCACCTCCATGAACAGGTC
Suggested primer 2:ACAAGCTGTACACCTACCGC