Insertion cassette: | CIB2 |
Side of cassette: | 3' |
Strand: | - |
Strain: | CLIP2.053369 |
Chromosome: | chromosome 16 |
Location: | 7443029 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g681238 | (1 of 1) K03650 - tRNA modification GTPase [EC:3.6.-.-] (mnmE, trmE, MSS1) | 5'UTR | |
Cre16.g681354 | (1 of 41) PF13920 - Zinc finger, C3HC4 type (RING finger) (zf-C3HC4_3) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGAGCTCCAGAGCGAACGCCCCCGTGAGAGCGCGGCTGGGGCTGGTAT |
Internal bar code: | ATACCACATGGGGGAAATCAAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2565 |
LEAP-Seq percent confirming: | 86.6667 |
LEAP-Seq n confirming: | 13 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCGGTGTGGTGAGGAATTGG |
Suggested primer 2: | CGCGTCAGCAATCCGTTAAG |