Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.053384 |
Chromosome: | chromosome 2 |
Location: | 7744687 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g146700 | (1 of 1) PF06747 - CHCH domain (CHCH) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGAAGCGAGGGCACACGGTTGGCCGGCATCTCCCATGCAAAGGCCGGG |
Internal bar code: | ACGGCGCGGAGACTGACAACTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 3410 |
LEAP-Seq percent confirming: | 85.2941 |
LEAP-Seq n confirming: | 29 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGTCGCCGTTGTACTTGGT |
Suggested primer 2: | CTCAGTGCTCCACGAGTGTT |