| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.053393 |
| Chromosome: | chromosome 12 |
| Location: | 8449076 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549950 | (1 of 1) IPR007305//IPR011691//IPR020846 - Vesicle transport protein, Got1/SFT2-like // Vesicle transport protein, SFT2-like // Major facilitator superfamily domain; SFT2-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGCTGCCAGCTGGCTGCCACATGTGTGCTCCAGGCCAGCGCAGCCCCC |
| Internal bar code: | AGCTAGGGAGTGTCTGCTGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 471 |
| LEAP-Seq percent confirming: | 22.2222 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGGAGGAGAAGCGCATC |
| Suggested primer 2: | GCGAGTTAAATCCGTGCGAC |