| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.053413 |
| Chromosome: | chromosome 2 |
| Location: | 4848315 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g109366 | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGCTGTGCAAACTCACCTGGGCGCCAGGAGCTCTCGGCCCAAGTAGC |
| Internal bar code: | TTTAACGTTCGTTAATATGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2647 |
| LEAP-Seq percent confirming: | 93.4066 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 91 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCAAATGTGAAAGCGGTG |
| Suggested primer 2: | GTGCCTGAGATTCCTGCCTT |