| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.053425 |
| Chromosome: | chromosome 8 |
| Location: | 341853 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358573 | MLO3 | transient receptor potential ion channel protein; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGAGCGCGGGTTGTGGCAGCAGCAGAAGCAGCCGGCTACGGCTGCACC |
| Internal bar code: | CAGTGGCCGTGTAATGACCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5474 |
| LEAP-Seq percent confirming: | 72.2222 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACCCAAACGCAGTCAAC |
| Suggested primer 2: | CTGGCTGTGGTGAGTGTCTT |