Insertion junction: CLIP2.053434_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.053434
Chromosome:chromosome 10
Location:1905379
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g431450 NAT24 N-acetyltransferase; (1 of 1) 2.3.1.1//2.3.1.88 - Amino-acid N-acetyltransferase / N-acetylglutamate synthase // Peptide alpha-N-acetyltransferase / Protein N-terminal acetyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCTACTCCAAACTGGGTTACCGGCTTGATGAGACGTCCCCCGACTATA
Internal bar code:TGTTGTGCCAACAGTGCTCTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2578
LEAP-Seq percent confirming:78.5714
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTCTCCTGCTCTGTGCTC
Suggested primer 2:CATGGCGGCCTTTTATGAGC