Insertion junction: CLIP2.053435_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.053435
Chromosome:chromosome 2
Location:1784367
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g086700 COG7 Component of oligomeric golgi complex; (1 of 1) PF10191 - Golgi complex component 7 (COG7) (COG7) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGAGCTGGGCCGGGGTGCGCTGGCGGAGGGGGCGGCGGACGGGCCGGC
Internal bar code:CTTGTGTGTCTGCTTTTGTGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:3609
LEAP-Seq percent confirming:74.5763
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:CAGACACTCAGCTGCTTGGA
Suggested primer 2:TGCCGTCCTTCAACACCTAC