| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.053440 |
| Chromosome: | chromosome 2 |
| Location: | 3689836 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g100350 | (1 of 2) PTHR31350:SF1 - F-BOX ONLY PROTEIN 21 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGGTGCTGTACTGCGCCGTGTGCCGGCGGCTGGGGCTGGCGGCGGTG |
| Internal bar code: | CGAGAGTTTGTGGAACCTTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 252 |
| LEAP-Seq percent confirming: | 6.57895 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 71 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCACACTACCACCTCCAC |
| Suggested primer 2: | GGAGCTCTCAAACCTTCGCA |