| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.053444 |
| Chromosome: | chromosome 10 |
| Location: | 4652894 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451800 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTGCGCAATAGCAGCCGCCAGCACTACTTTGACCTTGACATGTCAAAA |
| Internal bar code: | ATATAGGGGTAACGCAGTATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2397 |
| LEAP-Seq percent confirming: | 30.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATAGAGCCATCGCCGACTG |
| Suggested primer 2: | CTCCCCAGCTCCTACCTCTT |