Insertion junction: CLIP2.053447_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.053447
Chromosome:chromosome 1
Location:1451481
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g007737 FBT1 Folate/pteridine transporter 1; (1 of 1) IPR004324//IPR011701//IPR020846 - Folate-biopterin transporter // Major facilitator superfamily // Major facilitator superfamily domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGGGTTTGTGGGTGGATAGACACGCACACACACACAACCTCCGTGTGA
Internal bar code:GCGAGGGGGCCGGGTAAGGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:2773
LEAP-Seq percent confirming:90.2913
LEAP-Seq n confirming:93
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:103

Suggested primers for confirmation by PCR

Suggested primer 1:TCGCATAGATCGACCAACGG
Suggested primer 2:GGGTGGAGGGTAGCAGACTA