Insertion junction: CLIP2.053452_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.053452
Chromosome:chromosome 2
Location:1126301
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g080750 (1 of 274) IPR020683 - Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGAAGAAGCTGGAGTGAGAATTGGCGTGGCTTTGCGATGGGATGGAAG
Internal bar code:CCCGGGATCGCACCGGGAGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1427
LEAP-Seq percent confirming:94.7368
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:TTCACCCTGACACATCCTGC
Suggested primer 2:CTCTCATCTACCGCACCCAC