| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.053453 |
| Chromosome: | chromosome 14 |
| Location: | 991125 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613850 | (1 of 1) K12585 - exosome complex exonuclease DIS3/RRP44 (DIS3, RRP44) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAAGGGATTGTAAATACCAGCTCAAAACTAAAGCAAGCCGAGCTAAAG |
| Internal bar code: | TGCTGTTATGATAGTACGTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 70.5882 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACGCAACCGAACCCAATG |
| Suggested primer 2: | AACTGCTCTAGGGTTGGTGC |