| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.053470 |
| Chromosome: | chromosome 10 |
| Location: | 1133322 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425501 | (1 of 1) K03125 - transcription initiation factor TFIID subunit 1 (TAF1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCACCGCGCACACGGCGCCGGGGTCCATGTGTGCGCAAGCTAACGCGGG |
| Internal bar code: | TGAATTCGATGTAAGCTTATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 287 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTATGTGTGGACGCGTTCA |
| Suggested primer 2: | TGCGCAGTGTTTTCCTTGTG |