| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.053472 |
| Chromosome: | chromosome 2 |
| Location: | 5667776 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g115950 | (1 of 3) 3.2.1.39 - Glucan endo-1,3-beta-D-glucosidase / Laminarinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTCATGACGTTCCAGGAGGCGGCGCGGCTGCCGGCACGGAAGCGGCCT |
| Internal bar code: | AAACTTCTTACGTCTAGGACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1944 |
| LEAP-Seq percent confirming: | 76.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCCTCCCGCTTACTTATC |
| Suggested primer 2: | TGATACAGAGTTGCGCGGTT |