| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.053480 |
| Chromosome: | chromosome 3 |
| Location: | 6180769 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g191200 | RME3 | (1 of 3) K06236 - collagen, type I/II/III/V/XI/XXIV/XXVII, alpha (COL1AS); RNA methylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGATAGCCACGCGGCTCACCGGAACTCCCGCGGCAGCCCCCGCAGCAGC |
| Internal bar code: | GAATATTTGCAGGAAGGATGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 59 |
| LEAP-Seq percent confirming: | 1.72414 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGATGCCCTCATGCAGT |
| Suggested primer 2: | TCCGTACCTCCTGCTAGCTT |