| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.053494 |
| Chromosome: | chromosome 10 |
| Location: | 6528483 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g465400 | (1 of 1) IPR026305 - Negative elongation factor A | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTCTCCGGCCTCCTGACCTCTCTGGGCCTCCTGCGCCGCCCCGCCTCT |
| Internal bar code: | TCGTCATCACTGATCAATTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 387 |
| LEAP-Seq percent confirming: | 8.69565 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTCTGCAAAAGCCAGCAC |
| Suggested primer 2: | ATCTACACCCCCTCCTCCAC |