Insertion junction: CLIP2.053495_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.053495
Chromosome:chromosome 14
Location:3921041
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g632783 (1 of 1) K06634 - cyclin H (CCNH) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGCTCTGCCTCTCTTGCTTTGGCCGCTGCCCGCTCGCGCTTCTTGGCTT
Internal bar code:CAAGACTTACGTAGGTACAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:263
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CACCGCTACACACCAGTTCT
Suggested primer 2:ATTCAACAGGGGAGCTCAGC