Insertion junction: CLIP2.053497_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.053497
Chromosome:chromosome 6
Location:1586012
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g261350 (1 of 1) PF06108 - Protein of unknown function (DUF952) (DUF952) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAGTGGCAGGCGGGGATTTGTTCCGTATTATGGTCGCGGTATAAATATG
Internal bar code:GGTAGGGGATCGGCATCATCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:252
LEAP-Seq percent confirming:9.09091
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGTGTGGATCACTGGGAA
Suggested primer 2:ACACACCGGTACACGTACAC